Device

Part:BBa_K2074000:Design

Designed by: Tong Tong   Group: iGEM16_FAFU-CHINA   (2016-10-17)


Cry11Aa+2A+Cyt2 expressed in C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2850
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 2336
    Illegal NgoMIV site found at 2684
    Illegal NgoMIV site found at 2984
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704
    Illegal BsaI.rc site found at 899
    Illegal BsaI.rc site found at 2114
    Illegal SapI.rc site found at 2095


Design Notes

none


Source

HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry11Aa and Cyt2 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii.

References